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hag [2017-10-19 11:53:09]
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hag [2017-10-19 11:53:09]

flagellin protein, about 20,000 subunits make up one flagellum
locus
BSU35360
pI
4.78
mw
32.47 kDa
protein length
304 aa Sequence Blast
gene length
912 bp Sequence Blast
function
motility and chemotaxis
product
flagellin protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    3,634,987 → 3,635,901

    Phenotypes of a mutant

  • no swarming motility on B medium. [Pubmed|19202088]
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
  • few hours delay in pellicle development [Pubmed|26122431]
  • mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain [Pubmed|26122431]

    • The protein

    Protein family

  • bacterial flagellin family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|5A94391906ABFE70C5BA14E1E75343BC5805D0D9|YvzB] (C-terminal domain of Hag)

    • Structure

  • [PDB|3A5X] (from ''Salmonella typhimurium'', 42% identity)

    • [SW|Localization]

  • extracellular (no signal peptide), major component of the secretome [Pubmed|18957862]
  • membrane [Pubmed|18763711]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]
    description
    [Pubmed|2498284]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|2498284], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12730172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19118355], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

    • regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12730172]
    • view in new tab

    Other regulations

  • [protein|93F328524989597C7D2329B25E665496C9631E87|CsrA]: translation inhibition, [Pubmed|17555441]

    • Biological materials

    Mutant

  • GP901 (aphA3), GP902 (tet) [Pubmed|21856853], both available in [SW|Jörg Stülke]'s lab
  • 1A915 ( ''hag''::''cat''), [Pubmed|19202088], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC]
  • 1A783 ( ''hag''::''erm''), [Pubmed|2174860], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A783&Search=1A783 BGSC]
  • 1A842 ( ''hag''::''kan''), [Pubmed|14762011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A842&Search=1A842 BGSC]
  • DS1677 (marker-less in NCIB3610) [Pubmed|18566286]
  • BKE35360 (Δ[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTG
  • BKK35360 (Δ[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTG

    • Expression vector

  • expression in ''B. subtilis'', in [SW|pBQ200]: pGP1089, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP1035 (in [protein|search|pAC6]), pGP755 (in [protein|search|pAC7]), there is also a series of promoter deletion variants in [protein|search|pAC6] and [protein|search|pAC7] [Pubmed|21856853], all available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Daniel Kearns]

    • References

    Reviews

  • 22672726,25251856,26490009

    • Original Publications

  • 18566286,26122431,23144244,21602220,21856853,2498284,9648743,9096206,7708689,19202088,18763711,21895793,10809682,19118355,17555441,20534509,15378759