SubtiBank SubtiBank
bslA [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

bslA [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation
locus
BSU31080
pI
9.00
mw
19.00 kDa
function
biofilm formation, control of entry into sporulation via the phosphorelay
product
biofilm surface layer, inhibitor of KinA autophosphorylation
essential
no
synonyms
yuaB, sivB

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.3|Repellent surface layer]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation], [SW|phosphorelay]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|DegU regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,187,503 -> 3,188,048

    Phenotypes of a mutant

  • reduced colony complexity [Pubmed|21742882]
  • loss of surface repellency of the biofilm [Pubmed|22571672]
  • diminished [SW|sliding] of ''B. subtilis'' natto [Pubmed|26152584]
  • The protein

    Catalyzed reaction/ biological activity

  • inhibits [protein|search|KinA] autophophorylation [Pubmed|23335417]
  • contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms [Pubmed|26873313]
  • Paralogous protein(s)

  • [protein|search|SivA]
  • Structure

  • [PDB|4BHU] [Pubmed|23904481]
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • biofilm matrix in an extracellular polysaccharide-dependent manner [Pubmed|22571672]
  • forms a layer on the biofilm surface [Pubmed|22571672]
  • Expression and Regulation

    Operon

  • ''[protein|search|bslA]'' [Pubmed|22383849]
  • Regulatory mechanism

  • [protein|search|AbrB]: transcription repression [Pubmed|18978066]
  • [protein|search|LutR]: transcription activation [Pubmed|24196425]
  • expression is down-regulated in a ''[protein|search|rok]'' mutant (indirect effect) [Pubmed|21097620]
  • [protein|search|DegU]-P: transcription activation [Pubmed|18978066], this might be indirect [Pubmed|21742882]
  • Biological materials

    Mutant

  • MGNA-A218 (yuaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/218 NBRP B. subtilis, Japan]
  • ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) [Pubmed|17850253]
  • ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) [Pubmed|18978066]
  • ''B. subtilis'' ''bslA''::Cm (168 based) [Pubmed|21097620]
  • Expression vector

  • Physpank-bslA based on vector pDR111: pDRyuaB2 [Pubmed|21097620]
  • lacZ fusion

  • pNW500 P''bslA-lacZ'' fusion in pDG1663 [Pubmed|18978066]
  • GFP fusion

  • P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB [Pubmed|21097620]
  • References

    Reviews

  • 22607588,23979433,24988880,25907113,27458867
  • Original publications

  • 26152584,22571672,18978066,18957862,17850253,21097620,21742882,22383849,23335417,23904481,22571672,25870300,26152584,26378478,26873313