phoA [Sat Apr 30 2016 19:52:21 GMT+0200 (CEST)]

alkaline phosphatase A

locus: BSU09410

pI: 9.00

mw: 50.00 kDa

function: aquisition of phosphate upon phosphoate starvation

product: alkaline phosphatase A

essential: no

ec: 3.1.3.1

synonyms: phoAIV

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

[SW|Categories] containing this gene/protein

• [SW|phosphate metabolism]

This gene is a member of the following [SW|regulons]

• [SW|PhoP regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,017,083 -> 1,018,468

The protein

Catalyzed reaction/ biological activity

• A phosphate monoester H₂O = an alcohol phosphate (according to Swiss-Prot)

Protein family

• alkaline phosphatase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|PhoB]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operon

• ''phoA'' [Pubmed|16291680]

[SW|Sigma factor]

• SigA [Pubmed|8113174], [protein|search|SigG] [Pubmed|15699190]

Regulation

• expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680,8113174]

Regulatory mechanism

• [protein|search|PhoP]: transcription activation [Pubmed|16291680,8113174]

Biological materials

Mutant

• 1A767 ( ''phoA''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]

Labs working on this gene/protein

• [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

• 9593301,10913081,16491025,15699190,18957862,16291680,8113174,25666134