SubtiBank SubtiBank
prkD [2017-8-11 16:0:3]
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prkD [2017-8-11 16:0:3]

protein kinase D
locus
BSU02030
pI
6.36
mw
29.91 kDa
protein length
256 aa Sequence Blast
gene length
768 bp Sequence Blast
function
unknown
product
protein kinase D
essential
no
ec
2.7.11.1
synonyms
ybdM

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • Gene

    Coordinates
    223,219 → 223,989

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylates [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|DegS] on Ser-76 (in vitro) [Pubmed|21304896]
  • Protein family

  • [SW|protein kinase] domain (according to Swiss-Prot)
  • Modification

  • autophosphorylated on Thr-174 (''in vitro'') [Pubmed|20389117]
  • Biological materials

    Mutant

  • MGNA-B958 (ybdM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1957 NBRP B. subtilis, Japan]
  • GP578 (aphA3), available in the [SW|Stülke] lab
  • Expression vector

  • pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Stülke] lab, [pubmed|20389117]
  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Stülke] lab
  • lacZ fusion

  • pGP830 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References

  • 20389117,12406230,21304896,24731262,25278935