sftA

DNA translocase, translocation of non-segregated chromosomes prior to septum closure

locus: BSU_29805

pI: 5.00

mw: 107.00 kDa

protein length: 952 aa

gene length: 2859 bp

function: resolution of chromosome dimers

product: DNA translocase

essential: no

synonyms: ytpS,cdtA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

Gene

Coordinates: 3,049,725 → 3,052,583

The protein

Catalyzed reaction/ biological activity

• aids in moving DNA away from the closing septum, binds DNA, DNA-dependent ATPase activity [Pubmed|19818024]
• translocation of non-segregated chromosomes prior to septum closure
• the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|SftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]

Protein family

• FtsK/SpoIIIE/SftA family (with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE], according to UniProt)

Paralogous protein(s)

• [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE]

[SW|Domains]

• [SW|FtsK domain] (aa 622-813) (according to UniProt)

Structure

• [PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE]) [Pubmed|16916635]

[SW|Localization]

• 30% of the molecules in the cytoplasm (Homogeneous) [Pubmed|29439991,28110333,16479537]
• small fraction at the membrane [Pubmed|28110333]
• 70% of the molecules are septum-bound, co-localizes with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] at nascent division sites, recruitment to the septum depends on [protein|search|FtsA ][Pubmed|29439991,19788545]

Expression and Regulation

Operons

• genes: [gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]-[gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A438 (ytpS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/438 NBRP B. subtilis, Japan]
• 1A984 ( ''sftA''::''spec''), [Pubmed|19788545], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A984&Search=1A984 BGSC]
• BKE29805 (''[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
• BKK29805 (Δ[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC

References

Reviews

• 22047950,22934648

Original publications

• 29439991,16479537,19788545,19818024,21239579,16916635,27891124,28110333