ytcJ

putative amidohydrolase

locus: BSU_29550

pI: 5.46

mw: 58.31 kDa

protein length: 529 aa

gene length: 1590 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,022,068 → 3,023,657

The protein

Protein family

• [SW|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|3IGH] (from Pyrococcus horikoshii, 29% identity)

Expression and Regulation

Operons

• genes: [gene|48538F67259B25B64A7BC4263E944C7074E8E1B3|ytcI]-[gene|DF51B9E50BD6C87C5FD1A48E15F281FC3A096D0A|ytcJ]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A108 (ytcJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/108 NBRP B. subtilis, Japan]
• BKE29550 (Δ[gene|DF51B9E50BD6C87C5FD1A48E15F281FC3A096D0A|ytcJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCATTCACGTTCACTCTC, downstream forward: _UP4_TAAAAGGACAGGCAGCAGCC
• BKK29550 (Δ[gene|DF51B9E50BD6C87C5FD1A48E15F281FC3A096D0A|ytcJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCATTCACGTTCACTCTC, downstream forward: _UP4_TAAAAGGACAGGCAGCAGCC

References

• 9387221