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uvrB [2019-04-15 18:50:56]
excinuclease ABC (subunit B), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations
function
DNA repair after UV damage
product
excinuclease ABC (subunit B)
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]Gene
Coordinates
3,612,945 → 3,614,930
Phenotypes of a mutant
a [gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA] [gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB] mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]The protein
Protein family
uvrB family (according to Swiss-Prot)Structure
[PDB|2NMV] (bound to fluorescein-adducted DNA); [PDB|2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP [Pubmed|16426634][SW|Localization]
cytoplasm (according to Swiss-Prot)Biological materials
Mutant
GP1175 (del ''uvrAB''::''ermC'') (available in the [SW|Stülke] lab)BKE35170 (Δ[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAAATAAGCCTCCGTT, downstream forward: _UP4_CTAAAAGCGGAAGGATGATGBKK35170 (Δ[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAAATAAGCCTCCGTT, downstream forward: _UP4_CTAAAAGCGGAAGGATGATGReferences
Reviews
16464004,15927210,7801120,22933559 Original publications
8226626,9555905,16426634,21821766,24147116,21145481,25713353,30054368 
