SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

ykuC

similar to macrolide-efflux protein

locus: BSU_14030

pI: 9.92

mw: 47.93 kDa

protein length: 430 aa

gene length: 1293 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,475,150 → 1,476,442

Phenotypes of a mutant

• increased conjugation of ICEBs1 [Pubmed|25069588]

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE]-[gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]-[gene|DDAE68CD8D7F0D9C46E62D0EDBF4631C9EC4C236|ykuC]
  description: [Pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|11011148]

Biological materials

Mutant

• MGNA-A765 (ykuC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/765 NBRP B. subtilis, Japan]
• BKE14030 (Δ[gene|DDAE68CD8D7F0D9C46E62D0EDBF4631C9EC4C236|ykuC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACGCTCCTTACCC, downstream forward: _UP4_TAATGCAGGTTAAAAATGCG
• BKK14030 (Δ[gene|DDAE68CD8D7F0D9C46E62D0EDBF4631C9EC4C236|ykuC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACGCTCCTTACCC, downstream forward: _UP4_TAATGCAGGTTAAAAATGCG

References

• 22383849,25069588