ymaG

spore coat protein

locus: BSU_17310

pI: 9.68

mw: 13.64 kDa

protein length: 91 aa

gene length: 276 bp

function: protection of the spore

product: spore coat protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]

Gene

Coordinates: 1,865,512 → 1,865,787

The protein

[SW|Localization]

• inner spore coat [Pubmed|22171814,19933362]
• localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|DD441801394C75BA597D8029BED4CAD54ACCAD1C|ymaG]
  description: [Pubmed|12480901]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,12480901], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,12480901]

Biological materials

Mutant

• MGNA-B071 (ymaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1070 NBRP B. subtilis, Japan]
• BKE17310 (Δ[gene|DD441801394C75BA597D8029BED4CAD54ACCAD1C|ymaG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTTTCCTCCTGT, downstream forward: _UP4_TAAACGCAAAAGACCCCTTA
• BKK17310 (Δ[gene|DD441801394C75BA597D8029BED4CAD54ACCAD1C|ymaG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTTTCCTCCTGT, downstream forward: _UP4_TAAACGCAAAAGACCCCTTA

References

• 15699190,12480901,19933362,22171814,15383836