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holB [Fri Jan 29 2016 10:11:52 GMT+0100 (CET)]
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holB [Fri Jan 29 2016 10:11:52 GMT+0100 (CET)]

DNA polymerase III (delta subunit), part of the clamp-loader complex and the replisome
locus
BSU00310
pI
6.00
mw
37.00 kDa
function
DNA replication
product
DNA polymerase III (delta subunit)
essential
yes
ec
2.7.7.7
synonyms
yaaS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|DNA replication], [SW|essential genes]

    • Gene

    Coordinates on the chromosome (coding sequence)
    40,665 -> 41,654

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]

    • Structure

  • [PDB|1NJG] (from ''E. coli'', 36% identity)

    • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • cytoplasm (homogeneous) [Pubmed|16479537]

    • [SW|Interactions]

  • [protein|search|DnaX](3)-[protein|search|HolA]-[protein|search|HolB], the clamp-loader complex [Pubmed|23525462]
  • part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]

    • Expression and Regulation

    Operon

  • ''[protein|search|darA]-[protein|search|yaaR]-[protein|search|holB]-[protein|search|yaaT]-[protein|search|yabA]-[protein|search|yabB]-[protein|search|yazA]-[protein|search|yabC]'' [Pubmed|22383849]

    • Biological materials

    Expression vector

  • pGP1692: expression of Strep-''holB''-Strep in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP1698: expression of ''holB''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
  • pGP1699: expression of ''holB'' in ''E. coli'', in [SW|pGP574] (no tag due to a stop codon at the end of ''holB''), available in [SW|Jörg Stülke]'s lab
  • pGP2672: expression of ''holB''-Strep in ''B. subtilis'', in [SW|pGP380], suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 23268446,21958350,16479537,20122408,23525462,22383849