SubtiBank SubtiBank
holB
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

holB

DNA polymerase III (delta subunit), part of the clamp-loader complex and the [SW|replisome]
locus
BSU00310
pI
6.33
mw
37.47 kDa
protein length
329 aa Sequence Blast
gene length
987 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication]
product
DNA polymerase III (delta subunit)
essential
yes
ec
2.7.7.7
synonyms
yaaS

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    40,665 → 41,654

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Structure

  • [PDB|1NJG] (from ''E. coli'', 36% identity) [Pubmed|12623013]
  • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • cytoplasm (homogeneous) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|yabB]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
    description
    [Pubmed|22383849]

    regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase [Pubmed|23490197]
  • view in new tab

    Biological materials

    Expression vector

  • pGP1692: expression of Strep-''holB''-Strep in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP1698: expression of ''holB''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
  • pGP1699: expression of ''holB'' in ''E. coli'', in [SW|pGP574] (no tag due to a stop codon at the end of ''holB''), available in [SW|Jörg Stülke]'s lab
  • pGP2672: expression of ''holB''-Strep in ''B. subtilis'', in [SW|pGP380], suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • References

  • 23268446,21958350,16479537,20122408,23525462,22383849,12623013