zinT

lipoprotein, similar to zinc-binding protein

locus: BSU_26830

pI: 5.12

mw: 28.72 kDa

protein length: 251 aa

gene length: 756 bp

function: zinc-binding protein

product: zinc uptake

essential: no

synonyms: yrpE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,741,357 → 2,742,112

The protein

Protein family

• calycin superfamily (single member, according to UniProt)

Structure

• [PDB|1S7D] (the extracellular domain of E. coli ZinT, 46% identity)

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|DC6BA360289AFEC4E098AFDEE9C4E7FA02EA40F8|zinT]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|25649915], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

  regulation

  • induced by zinc starvation ([protein|search|Zur]) [Pubmed|25649915]

Biological materials

Mutant

• MGNA-A142 (yrpE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/142 NBRP B. subtilis, Japan]
• BKE26830 (Δ[gene|DC6BA360289AFEC4E098AFDEE9C4E7FA02EA40F8|zinT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAACACTCCTTTTTT, downstream forward: _UP4_TAAATGATGATTACTCTATG
• BKK26830 (Δ[gene|DC6BA360289AFEC4E098AFDEE9C4E7FA02EA40F8|zinT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAACACTCCTTTTTT, downstream forward: _UP4_TAAATGATGATTACTCTATG

References

• 12904577,25649915,18957862,27561249,20097857