SubtiBank SubtiBank
hutH [Mon Aug 31 2015 13:57:38 GMT+0200 (CEST)]
SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

hutH [Mon Aug 31 2015 13:57:38 GMT+0200 (CEST)]

histidase
locus
BSU39350
pI
5.22
mw
55.00 kDa
function
histidine utilization
product
histidase
essential
no
ec
4.3.1.3
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.5|Utilization of histidine]
  • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CodY regulon], [SW|HutP regulon]
  • The protein

    Catalyzed reaction/ biological activity

  • L-histidine = urocanate + NH3 (according to Swiss-Prot)
  • Protein family

  • PAL/histidase family (according to Swiss-Prot)
  • Structure

  • [PDB|1B8F] (from ''Pseudomonas putida'', 43% identity, 62% similarity) [Pubmed|10220322]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operon

  • ''[protein|search|hutP]-[protein|search|hutH]-[protein|search|hutU]-[protein|search|hutI]-[protein|search|hutG]-[protein|search|hutM]'' [Pubmed|10746760]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8071225]
  • Regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|8071225]
  • induced by histidine ([protein|search|HutP]) [Pubmed|8071225]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|8682780]
  • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|8071225]
  • [protein|search|CodY]: transcription repression [Pubmed|8682780]
  • [protein|search|HutP]: transcriptional antitermination at a protein-dependent [SW|RNA switch] located between ''[protein|search|hutP]'' and ''[protein|search|hutH]'' [Pubmed|8071225]
  • Biological materials

    Mutant

  • GP1114 (spc), available in [SW|Stülke] lab
  • References

    Reviews

  • 22933560
  • Original publications

  • 10746760,6143742,8071225,8682780,10217782