hutH [Mon Aug 31 2015 13:57:38 GMT+0200 (CEST)]

histidase

locus: BSU39350

pI: 5.22

mw: 55.00 kDa

function: histidine utilization

product: histidase

essential: no

ec: 4.3.1.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.5|Utilization of histidine]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|CodY regulon], [SW|HutP regulon]

The protein

Catalyzed reaction/ biological activity

• L-histidine = urocanate + NH₃ (according to Swiss-Prot)

Protein family

• PAL/histidase family (according to Swiss-Prot)

Structure

• [PDB|1B8F] (from ''Pseudomonas putida'', 43% identity, 62% similarity) [Pubmed|10220322]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|hutP]-[protein|search|hutH]-[protein|search|hutU]-[protein|search|hutI]-[protein|search|hutG]-[protein|search|hutM]'' [Pubmed|10746760]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8071225]

Regulation

• repressed by glucose ([protein|search|CcpA]) [Pubmed|8071225]
• induced by histidine ([protein|search|HutP]) [Pubmed|8071225]
• repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|8682780]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression [Pubmed|8071225]
• [protein|search|CodY]: transcription repression [Pubmed|8682780]
• [protein|search|HutP]: transcriptional antitermination at a protein-dependent [SW|RNA switch] located between ''[protein|search|hutP]'' and ''[protein|search|hutH]'' [Pubmed|8071225]

Biological materials

Mutant

• GP1114 (spc), available in [SW|Stülke] lab

References

Reviews

• 22933560

Original publications

• 10746760,6143742,8071225,8682780,10217782