yhcR

extracellular non-specific endonuclease, RNase

Locus: BSU_09190

Isoelectric point: 4.69

Molecular weight: 132.47 kDa

Protein length: 1217 aa

Gene length: 3654 bp

Function: utilization of nucleic acids

Product: extracellular non-specific endonuclease, RNase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
• Information processing → RNA synthesis and degradation → RNases → Endoribonucleases
• Groups of genes → Secreted proteins

Gene

Coordinates: 991,348 → 995,001

The protein

Protein family

• 5'-nucleotidase family (with YfkN, according to UniProt)

Paralogous protein(s)

• YfkN

Domains

• TNase-like domain (aa 376-517) (according to UniProt)

Structure

• 2Z1A (from Thermus thermophilus, corresponds to aa 590 ... 1094, 29% identity)

Localization

• extracellular (signal peptide) PubMed, attached to the cell wall PubMed

Expression and Regulation

Operons

• Genes: yhcR-yhcS
  Description: PubMed

Biological materials

Mutant

• MGNA-A665 (yhcR::erm), available at the NBRP B. subtilis, Japan
• BKE09190 (ΔyhcR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAATTCCTTTCAGGCT, downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG
• BKK09190 (ΔyhcR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAATTCCTTTCAGGCT, downstream forward: _UP4_AGAAAAAGGAGCGCCTCCAG

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

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Original Publications

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