SubtiBank SubtiBank
ackA

ackA

acetate kinase
locus
BSU29470
pI
5.19
mw
42.98 kDa
protein length
395 aa Sequence Blast
gene length
1185 bp Sequence Blast
function
overflow metabolism
product
acetate kinase
essential
no
ec
2.7.2.1
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
  • Gene

    Coordinates
    3,015,111 → 3,016,298

    The protein

    Catalyzed reaction/ biological activity

  • ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)
  • Protein family

  • acetokinase family (according to Swiss-Prot)
  • Structure

  • [PDB|2IIR] (from ''Thermotoga maritima'', 54% identity, 73% similarity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]
    description
    [Pubmed|8226682]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8226682], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|16995897], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|8226682,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • regulation

  • activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|16995897]
  • view in new tab

    Biological materials

    Mutant

  • GP1902 (''ackA''::''aphA3'') (available in [SW|Jörg Stülke]'s lab)
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • References

    Reviews

  • 16678422,15755952
  • Original publications

  • 8226682,9811655,15378759,11244084,16995897,12850135,8226682,9811655,21106498,26098117