ackA

acetate kinase

locus: BSU_29470

pI: 5.19

mw: 42.98 kDa

protein length: 395 aa

gene length: 1188 bp

function: overflow metabolism

product: acetate kinase

essential: no

ec: 2.7.2.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]

Gene

Coordinates: 3,015,111 → 3,016,298

The protein

Catalyzed reaction/ biological activity

• acetate + ATP --> acetyl phosphate + ADP (according to UniProt)

Protein family

• acetokinase family (with [protein|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|Buk], according to UniProt)

Structure

• [PDB|2IIR] (from ''Thermotoga maritima'', 54% identity, 73% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]
  description: [Pubmed|8226682]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8226682], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|16995897], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|8226682,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • activated during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|16995897]

Biological materials

Mutant

• GP1902 (''ackA''::''aphA3'') (available in [SW|Jörg Stülke]'s lab)
• BKE29470 (Δ[gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT
• BKK29470 (Δ[gene|DAA285C86692E8B47D48652E0973AE3FF091CBC3|ackA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTGACGCTCCTTTAT, downstream forward: _UP4_TAAATCGCATGAAAGCACAT

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

References

Reviews

• 16678422,15755952

Original publications

• 8226682,9811655,15378759,11244084,16995897,12850135,8226682,9811655,21106498,26098117