SubtiBank SubtiBank
murAA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

murAA

UDP-N-acetylglucosamine 1-carboxyvinyltransferase
locus
BSU36760
pI
5.45
mw
46.54 kDa
protein length
436 aa Sequence Blast
gene length
1308 bp Sequence Blast
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
essential
yes
ec
2.5.1.7
synonyms
murA

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,777,949 → 3,779,259

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Phosphoenolpyruvate UDP-N-acetyl-D-glucosamine = phosphate UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)
  • Protein family

  • MurA subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|523B01B5E7141C7DFC28CE67EB92580F49C20844|MurAB]
  • Structure

  • [PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA] is subject to [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]-dependent degradation upon glucose starvation [Pubmed|14763982,17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|32CE4F77F08F926152BECAB56E2841E1FFB27600|ywzB]-[gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|26735940], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]
  • view in new tab

    genes
    [gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
    description
    [Pubmed|9353933]

    regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]
  • view in new tab

    genes
    [gene|3A8E0417C4EC85FDDE1178B775F6579C8F1009C6|spoIIT]-[gene|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
    description
    [Pubmed|26735940]

    regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|26735940]
  • view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
  • Biological materials

    Expression vector

  • pRSETA-murAA, Expression of His(6)-MurAA, avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]
  • pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]
  • References

    Reviews

  • 21822642
  • Original publications

  • 14763982,18063720,19899805,14763982,8994972,9485407,26735940,22383849,26883633