yqhS

similar to 3-dehydroquinate dehydratase

locus: BSU_24470

pI: 5.12

mw: 16.29 kDa

protein length: 148 aa

gene length: 447 bp

function: unknown

product: unknown

essential: no

ec: 4.2.1.10

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,539,755 → 2,540,201

The protein

Catalyzed reaction/ biological activity

• 3-dehydroquinate --> 3-dehydroshikimate + H₂O (according to UniProt)

Protein family

• type-II 3-dehydroquinase family (single member, according to UniProt)

Structure

• [PDB|1GQO]

Expression and Regulation

Operons

• genes: [gene|449EEBF33F52B461F244331ACD140E68CF64EE7C|yqhR]-[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
  description: [pubmed|22383849]

• genes: [gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C465 (yqhS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2463 NBRP B. subtilis, Japan]
• CS214 (''[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE24470 (Δ[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACAATCTCTCCTTTTCG, downstream forward: _UP4_AGCCAGCAAGGGGGAGAATC
• BKK24470 (Δ[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACAATCTCTCCTTTTCG, downstream forward: _UP4_AGCCAGCAAGGGGGAGAATC

References

• 27197833