SubtiBank SubtiBank
epsL
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epsL

similar to UDP-galactose phosphate transferase, extracellular polysaccharide synthesis
locus
BSU_34250
pI
9.97
mw
22.93 kDa
protein length
202 aa Sequence Blast
gene length
609 bp Sequence Blast
function
biofilm formation
product
unknown
essential
no
synonyms
yvfC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,516,880 → 3,517,488

    The protein

    Protein family

  • Bacterial sugar transferase family (with [protein|search|TuaA], according to UniProt)

    • Paralogous protein(s)

  • [protein|4AC72C556ED02A7DE4E1E8D69A0FC926359ACE28|TuaA/1], [protein|5790E3DB39F31017B557C2D3F428C0348A461DA6|TuaA/2]

    • Structure

  • [PDB|5W7L] (from Campylobacter concisus, 58% identity) [pubmed|29769739]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]
    description
    [Pubmed|15661000]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15661000], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: anti-activation, (prevents binding of [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]) [Pubmed|23646920], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [regulon|EAR riboswitch|EAR riboswitch]: processive antitermination, in [regulon|EAR riboswitch|EAR riboswitch]

    • regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|15661000]

    • additional information

  • induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI] or [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] [PubMed|15661000,19788541]
  • expression is increased in [gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX] mutants [pubmed|32483306]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A065 (yvfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/65 NBRP B. subtilis, Japan]
  • BKE34250 (Δ[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA, downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC
  • BKK34250 (Δ[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA, downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC

    • labs

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

    • References

    Reviews

  • 20735481

    • Research papers

  • 29769739

    • The EAR [SW|RNA switch]

  • 20374491,20230605