yacC [2019-06-04 16:24:55]

similar to chaperonin (HSP33 Homolog), putative disulfide bond chaperone

locus: BSU00710

pI: 4.66

mw: 31.66 kDa

protein length: 291 aa

gene length: 876 bp

function: unknown

product: putative disulfide bond chaperone

essential: no

synonyms: hslO

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.3|Chaperone/ protein folding/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 79,880 → 80,755

The protein

Protein family

• HSP33 family (according to Swiss-Prot)

Structure

• [PDB|1VZY]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]-[gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]-[gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30480837], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by heat stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30480837]

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]
  description: [Pubmed|18179421]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

Biological materials

Mutant

• MGNA-B924 (yacC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1923 NBRP B. subtilis, Japan]
• BKE00710 (Δ[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAATAATCCATTACTAAAC, downstream forward: _UP4_TAAGCTCTTTAGCGGGTTTT
• BKK00710 (Δ[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAATAATCCATTACTAAAC, downstream forward: _UP4_TAAGCTCTTTAGCGGGTTTT

References

Research papers

• 28516784,30480837