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walJ [2017-8-11 16:0:3]
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walJ [2017-8-11 16:0:3]

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication
locus
BSU40370
pI
5.50
mw
29.77 kDa
protein length
268 aa Sequence Blast
gene length
804 bp Sequence Blast
function
coordination of cell division with DNA replication
product
5'-3' double-stranded DNA exonuclease
essential
no
ec
3.-.-.-
synonyms
yycJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]

    • Gene

    Coordinates
    4,148,851 → 4,149,645

    Phenotypes of a mutant

  • the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited [Pubmed|21169496]
  • increased sensitivity to a narrow spectrum of cephalosporin antibiotics [Pubmed|21169496]
  • disruption of the corresponding gene in B. anthracis results in a mutator phenotype [Pubmed|21205011]

    • The protein

    Catalyzed reaction/ biological activity

  • 5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA [Pubmed|23491602]
  • the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of [SW|cell division] with DNA replication [Pubmed|21169496]

    • Protein family

  • predicted member of the metallo-β-lactamase superfamily

    • [SW|Cofactors]

  • Mn(II) [Pubmed|23491602]

    • Expression and Regulation

    Operons

    genes
    [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
    description
    [Pubmed|9829949]

    regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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    Biological materials

    Mutant

  • MGNA-B824 (yycJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1823 NBRP B. subtilis, Japan]
  • 1A1044 ( ''walJ''::''spec''), [Pubmed|21169496], available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]
  • BKE40370 (''walJ''::''erm trpC2''), available at [http://bgsc.org] [pubmed|28189581]

    • References

    Reviews

  • 26343983

    • Original publications

  • 9829949,21169496,21205011,23491602