SubtiBank SubtiBank
walJ [Tue Jan 03 2017 17:43:37 GMT+0100 (CET)]
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walJ [Tue Jan 03 2017 17:43:37 GMT+0100 (CET)]

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication
locus
BSU40370
pI
5.00
mw
29.00 kDa
function
coordination of cell division with DNA replication
product
5'-3' double-stranded DNA exonuclease
essential
no
ec
3.-.-.-
synonyms
yycJ

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)]
  • [SW|Categories] containing this gene/protein

  • [SW|cell division], [SW|cell wall synthesis], [SW|DNA repair/ recombination]
  • Gene

    Coordinates on the chromosome (coding sequence)
    4,148,851 -> 4,149,645

    Phenotypes of a mutant

  • the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited [Pubmed|21169496]
  • increased sensitivity to a narrow spectrum of cephalosporin antibiotics [Pubmed|21169496]
  • disruption of the corresponding gene in B. anthracis results in a mutator phenotype [Pubmed|21205011]
  • The protein

    Catalyzed reaction/ biological activity

  • 5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA [Pubmed|23491602]
  • the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of [SW|cell division] with DNA replication [Pubmed|21169496]
  • Protein family

  • predicted member of the metallo-β-lactamase superfamily
  • [SW|Cofactors]

  • Mn(II) [Pubmed|23491602]
  • Expression and Regulation

    Operon

  • ''[protein|search|walR]-[protein|search|walK]-[protein|search|yycH]-[protein|search|yycI]-[protein|search|walJ]-[protein|search|htrC]'' [Pubmed|9829949]
  • Regulation

  • expressed during vegetative growth, repressed during stationary phase [Pubmed|9829949]
  • Biological materials

    Mutant

  • MGNA-B824 (yycJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1823 NBRP B. subtilis, Japan]
  • 1A1044 ( ''walJ''::''spec''), [Pubmed|21169496], available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]
  • BKE40370 (''walJ''::''erm trpC2''), available at [http://bgsc.org]
  • References

    Reviews

  • 26343983
  • Original publications

  • 9829949,21169496,21205011,23491602