SubtiBank SubtiBank
ykfC
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ykfC

D-glutamyl-L-amino acid peptidase
Locus
BSU_12990
Isoelectric point
6.35
Molecular weight
32.91 kDa
Protein length
296 aa Sequence Blast
Gene length
891 bp Sequence Blast
Function
cell wall metabolism
Product
D-glutamyl-L-amino acid peptidase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall/ other

    • Gene

    Coordinates
    1,367,941 → 1,368,831

    The protein

    Catalyzed reaction/ biological activity

  • cleaves L-Ala-γ-D-Glu dipeptides from cell wall peptides PubMed
  • The enzyme releases L-Ala-gamma-D-Glu dipeptides from cell wall peptides via cleavage of an L-Ala-gamma-D-Glu-|-L-Lys bond (according to UniProt)

    • Protein family

  • Peptidase C40 family (according to UniProt)

    • Structure

  • 3H41 (from B. cereus, 39% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD
    Description
    PubMed

    Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
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    Biological materials

    Mutant

  • MGNA-A742 (ykfC::erm), available at the NBRP B. subtilis, Japan
  • BKE12990 (ΔykfC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGCTGATATGACAGTGT, downstream forward: _UP4_TTTTCAGAATAAGGAGGGAG
  • BKK12990 (ΔykfC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTGCTGATATGACAGTGT, downstream forward: _UP4_TTTTCAGAATAAGGAGGGAG

    • References

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