natR

two-component response regulator, regulation of the [gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]-[gene|search|natB ]operon

locus: BSU_02740

pI: 6.61

mw: 26.82 kDa

protein length: 233 aa

gene length: 702 bp

function: regulation of the [gene|B019FD0CB6F76C03DA5E76ABD1B324BA5E1A6936|natA]-[gene|search|natB ]operon

product: two-component response regulator

essential: no

synonyms: yccH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.2|Sodium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 295,584 → 296,285

The protein

Protein family

• OmpR family of two-component response regulators

[SW|Domains]

• [SW|Response regulatory domain] (aa 3-117) (according to UniProt)
• HTH LytTR-type domain (aa 129-233) (according to UniProt)

Modification

• phosphorylated by [protein|A4644CB18F7D0DF3E7E50ABB0C0F224D5744A1A5|NatK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|5HEV] (from Enterococcus faecium, corresponds to aa 1 ... 172, 22.3% identity) [pubmed|27670715]

[SW|Localization]

• cytoplasm (according to UniProt)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000232 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|D3F6337B94A633730E514B05CA852BE9AA3741B3|natR]-[gene|A4644CB18F7D0DF3E7E50ABB0C0F224D5744A1A5|natK]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C043 (yccH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2041 NBRP B. subtilis, Japan]
• BKE02740 (Δ[gene|D3F6337B94A633730E514B05CA852BE9AA3741B3|natR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCATTCCCCTAGTTA, downstream forward: _UP4_TACTATTTTTGAGGAGTTTA
• BKK02740 (Δ[gene|D3F6337B94A633730E514B05CA852BE9AA3741B3|natR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCATTCCCCTAGTTA, downstream forward: _UP4_TACTATTTTTGAGGAGTTTA

References

• 10094672,17322186,27670715