two-component response regulator, regulation of degradative enzyme expression, genetic competence, biofilm formation, capsule biosynthesis (together with SwrAA), non-phosphorylated DegU is required for swarming motility
function
regulation of degradative enzymes, genetic competence,
product
two-component response regulator
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue][SW|Categories] containing this gene/protein
[SW|biofilm formation], [SW|transcription factors and their control], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|DegU regulon], [SW|SinR regulon], [SW|TnrA regulon]Gene
Coordinates on the chromosome (coding sequence)
3,644,607 -> 3,645,296
Phenotypes of a mutant
defect in [SW|biofilm formation] [Pubmed|20815827], this can be suppressed by [protein|search|DegU]-independent expression of [protein|search|BslA] [Pubmed|21742882]the mutation suppresses the mucoid phenotype of ''[protein|search|motA]'' or ''[protein|search|motB]'' mutants due to reduced expression of the ''[protein|search|capB]-[protein|search|capC]-[protein|search|capA]-[protein|search|capE]'' operon [Pubmed|24296669]The protein
Catalyzed reaction/ biological activity
transcription activator and repressor (see [SW|DegU regulon])DegU-P represses expression of the fla/che operon (''[protein|search|flgB]-[protein|search|flgC]-[protein|search|fliE]-[protein|search|fliF]-[protein|search|fliG]-[protein|search|fliH]-[protein|search|fliI]-[protein|search|fliJ]-[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'') in the absence of [protein|search|SwrA], and switches to an activator of the operon in the presence of [protein|search|SwrA] [Pubmed|24386445]Modification
phosphorylated by [protein|search|DegS] on Asp-56 [Pubmed|1901568], this modulates DNA-binding activityphosphorylation by [protein|search|DegS] occurs in response to inhibition of flagellar rotation [Pubmed|23888912]Effectors of protein activity
DNA-binding activity of DegU is inhibited by [protein|search|RapG] [Pubmed|12950930][protein|search|DegU]-P is degraded by [protein|search|ClpC]-[protein|search|ClpP] [Pubmed|20070525]Structure
[PDB|4GVP] (VraR from ''Staphylococcus aureus'', 35% identity) [Pubmed|23650349][SW|Localization]
cytoplasm (according to Swiss-Prot)[SW|Interactions]
[protein|search|DegU]-[protein|search|RapG] [Pubmed|12950930][protein|search|DegS]-[protein|search|DegU][protein|search|SwrA]-[protein|search|DegU]-P (N-terminal domain) [Pubmed|24386445,22496484]Additional information
[protein|search|DegU]-P is degraded by [protein|search|ClpC]-[protein|search|ClpP] [Pubmed|20070525]accumulation of [protein|search|DegU]-P results in decreased transcription of the ''[protein|search|epsA]-[protein|search|epsO]'' and ''[protein|search|tapA]-[protein|search|sipW]-[protein|search|tasA]'' operons due to increased levels of [SW|Spo0A]-P [Pubmed|24123822]Expression and Regulation
Operon
''[protein|search|degS]-[protein|search|degU]'' [Pubmed|1688843]''[protein|search|degU]'' [Pubmed|18502860][SW|Sigma factor]
''[protein|search|degS]'': [protein|search|SigA] [Pubmed|1688843]''[protein|search|degU]'': [protein|search|SigA] [Pubmed|18502860]Regulation
induced in the presence of ammonium ([protein|search|TnrA]) [Pubmed|18502860]induced by glucose ([protein|search|CcpA]) [Pubmed|23123903]Regulatory mechanism
[protein|search|DegU]: positive autoregulation [Pubmed|18502860][protein|search|TnrA]: transcription repression [Pubmed|18502860][protein|search|CcpA]: transcription activation [Pubmed|23123903][protein|search|SinR]: transcription repression [Pubmed|24317403]Additional information
[protein|search|DegU]-P is degraded by [protein|search|ClpC]-[protein|search|ClpP] [PubMed|20070525]Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s labReferences
Reviews
20030732,19118340,23927648,24988880 The [SW|DegU regulon]
Original Publications
17850253,8878039,14563871,1901568,1321152,1459944,18194340,18978066,10908654,18502860,10094672,19389763,20815827,21742882,18414485,19420703,2428811,7746142,18502860,19389763,19416356,19734658,1688843,20070525,18197985,15598897,12950930,17590234,8955341,22496484,22745669,23123903,21965392,24123822,24296669,122328658,23888912,24149708,24317403,25431404,25433860,24386445,25777015,25880922,25887289,25755103,26819068, 23650349,27026185 
