SubtiBank SubtiBank
yxiM
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yxiM

similar to rhamnogalacturonan acetylesterase
locus
BSU_39120
pI
6.62
mw
41.68 kDa
protein length
382 aa Sequence Blast
gene length
1149 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    4,017,508 → 4,018,656

    The protein

    Protein family

  • [SW|'GDSL' lipolytic enzyme family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|578B79EB64B804FCAACA73AB22458481371A815C|YesY], [protein|585CA3154C0405697C4707E5B1D9359963999C54|YesT]
  • Structure

  • [PDB|2O14]
  • Expression and Regulation

    Operons

    genes
    [gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
    description
    [Pubmed|10746760]

    regulation

  • expression declines in the stationary phase [Pubmed|23175651]
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    Biological materials

    Mutant

  • MGNA-B719 (yxiM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1718 NBRP B. subtilis, Japan]
  • GP1116 (spc), available in [SW|Stülke] lab
  • BKE39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA
  • BKK39120 (Δ[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGACTCCCCCTCTAT, downstream forward: _UP4_TAATACATCATTTTTCGACA