ysxE

inner spore coat protein

locus: BSU_28100

pI: 5.64

mw: 40.41 kDa

protein length: 341 aa

gene length: 1026 bp

function: protection of the spore

product: inner spore coat protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.3|Class III]

Gene

Coordinates: 2,869,964 → 2,870,989

The protein

[SW|Localization]

• inner spore coat, localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID]-[gene|D1EAEC9C88EDDD8FB2E7E315DE28A7A54AAAB4D2|ysxE]
  description: [Pubmed|15699190,8449878]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,8449878], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,8449878]

Biological materials

Mutant

• MGNA-A997 (ysxE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/997 NBRP B. subtilis, Japan]
• BKE28100 (Δ[gene|D1EAEC9C88EDDD8FB2E7E315DE28A7A54AAAB4D2|ysxE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTCACCACATTTT, downstream forward: _UP4_TCTTAATGGCGCGGACTTGT
• BKK28100 (Δ[gene|D1EAEC9C88EDDD8FB2E7E315DE28A7A54AAAB4D2|ysxE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTCACCACATTTT, downstream forward: _UP4_TCTTAATGGCGCGGACTTGT

References

• 15699190,8449878,12107147,22171814