yerD [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

general stress protein, similar to glutamate synthase (ferredoxin), required for protection against paraquat stress

locus: BSU06590

pI: 7.00

mw: 58.00 kDa

function: protection against paraquat stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

[SW|Categories] containing this gene/protein

• [SW|general stress proteins (controlled by SigB)], [SW|resistance against oxidative and electrophile stress], [SW|poorly characterized/ putative enzymes], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigB regulon]

Gene

Coordinates on the chromosome (coding sequence): 716,780 -> 718,357

The protein

Paralogous protein(s)

• [protein|search|GltA]

[SW|Cofactors]

• FMN or FAD [Pubmed|21635694]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operon

• ''[protein|search|yerD]'' [Pubmed|22383849]

[SW|Sigma factor]

• [protein|search|SigB] [Pubmed|15805528]

Regulation

• induced by stress ([protein|search|SigB]) [Pubmed|15805528]
• strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]

Biological materials

Mutant

• GP1184 (tet) available in [SW|Jörg Stülke]'s lab

Expression vector

• pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein, based on [SW|pGP172]), available in [SW|Fabian Commichau]'s lab

FLAG-tag construct

• GP1187 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Fabian Commichau] University of Göttingen, Germany
• [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

References

• 15805528,22383849,23033921,22582280