ilvA [Mon May 16 2016 16:52:54 GMT+0200 (CEST)]

threonine dehydratase

locus: BSU21770

pI: 5.00

mw: 46.00 kDa

function: biosynthesis of branched-chain amino acids

product: threonine dehydratase

essential: no

ec: 4.3.1.19

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|CodY regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,292,769 -> 2,294,037

The protein

Catalyzed reaction/ biological activity

• L-threonine = 2-oxobutanoate NH₃ (according to Swiss-Prot)

Protein family

• ccmF/cycK/ccl1/nrfE/ccsA family (according to Swiss-Prot)

Structure

• [PDB|1TDJ] (the enzyme from ''E. coli'', 39% identity) [Pubmed|9562556]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

Expression and Regulation

Operon

• ''[protein|search|ilvA]-[protein|search|ypmP]'' [Pubmed|15060025]

Regulation

• repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|12618455]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

Biological materials

lacZ fusion

• pGP519 (in [protein|search|pAC5]), available in [SW|Stülke] lab

References

• 18083814,12618455,15060025,9562556,12618455,12107147,24163341,26220295