azoR1

azoreductase, involved in quinone detoxification

locus: BSU_19230

pI: 4.99

mw: 22.83 kDa

protein length: 208 aa

gene length: 627 bp

function: quinone detoxification

product: azoreductase

essential: no

synonyms: yocJ

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,096,350 → 2,096,976

The protein

Catalyzed reaction/ biological activity

• anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD⁺ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H⁺ + 2 NADH (according to UniProt)

Protein family

• azoreductase type 1 family (with [protein|0E2B1914A040666BAF042C0BE3F468144E1F1E06|AzoR2], according to UniProt)

Paralogous protein(s)

• [protein|0E2B1914A040666BAF042C0BE3F468144E1F1E06|AzoR2]

Structure

• [PDB|3P0R] (from B. anthracis, 64% identity, 81% similarity)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB]: repression, [pubmed|18208493], in [regulon|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB regulon]

  regulation

  • induced by thiol-reactive compounds (catechol, menaquinone) ([protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB]) [pubmed|18208493]

Biological materials

Mutant

• MGNA-B415 (yocJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1414 NBRP B. subtilis, Japan]
• BKE19230 (Δ[gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA
• BKK19230 (Δ[gene|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|azoR1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA

References

• 18208493,17725564,15378759