SubtiBank SubtiBank
ispD
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ispD

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00900
pI
5.58
mw
25.70 kDa
protein length
232 aa Sequence Blast
gene length
699 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
essential
yes
ec
2.7.7.60
synonyms
yacM

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    109,789 → 110,487

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-C-methyl-D-erythritol 4-phosphate + CTP + H+ --> 4-CDP-2-C-methyl-D-erythritol + diphosphate (according to UniProt)
  • Protein family

  • cytidylyltransferase family (with [protein|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|TagD] and [protein|B25AE6979AF12628780C4489FFED966C6B31968A|YfnH], according to UniProt)
  • IspD/TarI cytidylyltransferase family (single member, according to UniProt)
  • Structure

  • [PDB|5DDT] [Pubmed|27821871]
  • [PDB|5DDV] [Pubmed|27821871]
  • Expression and Regulation

    Operons

    genes
    [gene|179FDEA51A060DF32A3EA06A796793DE27B600FF|yacL]-[gene|CF9633F769263C6D16EE778F6BD2ECF1995F512A|ispD]-[gene|1A6793A3A5AC568C67873C8708E9F581F42AB9BA|ispF]
    description
    [Pubmed|10482513]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
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    Biological materials

    Mutant

  • MGNA-B881 (yacM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1880 NBRP B. subtilis, Japan]
  • References

  • 15805528,10482513,17458547,17434969,23840410,21766403,27821871,29335298