SubtiBank SubtiBank
prmC
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prmC

similar to N5-glutamine methyltransferase that modifies peptide release factors
locus
BSU_37000
pI
4.89
mw
32.23 kDa
protein length
288 aa Sequence Blast
gene length
867 bp Sequence Blast
function
peptide release factor methylation
product
N5-glutamine methyltransferase
essential
no
ec
2.1.1.297
synonyms
hemK, ywkE

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.13|Translation/ other/ based on similarity]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.8|Protein modification/ other]
  • Gene

    Coordinates
    3,796,217 → 3,797,083

    Phenotypes of a mutant

  • non-transformable [pubmed|28189581]
  • The protein

    Catalyzed reaction/ biological activity

  • L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine --> H+ + N5-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine (according to UniProt)
  • Protein family

  • protein N5-glutamine methyltransferase family (single member, according to UniProt)
  • Structure

  • [PDB|1NV8] (the protein from ''Thermotoga maritima'') [Pubmed|12741815]
  • Expression and Regulation

    Operons

    genes
    [gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A199 (ywkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/199 NBRP B. subtilis, Japan]
  • BKE37000 (Δ[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGGGCTTCGAATATCGTCT, downstream forward: _UP4_TAAGCAGCTTGCTGGCGAGT
  • BKK37000 (Δ[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAGGGCTTCGAATATCGTCT, downstream forward: _UP4_TAAGCAGCTTGCTGGCGAGT
  • References

  • 11805295,11847124,12741815,28189581