SubtiBank SubtiBank
relA [Thu Nov 24 2016 18:04:42 GMT+0100 (CET)]
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relA [Thu Nov 24 2016 18:04:42 GMT+0100 (CET)]

GTP pyrophosphokinase (stringent response)
locus
BSU27600
pI
6.00
mw
84.00 kDa
function
synthesis and degradation of (p)ppGpp,
triggering the stringent response
product
GTP pyrophosphokinase
essential
no
ec
2.7.6.5
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [SW|Categories] containing this gene/protein

  • [SW|metabolism of signalling nucleotides]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,820,529 -> 2,822,733

    Phenotypes of a mutant

  • requirement for valine [Pubmed|24163341]
  • a ''[protein|search|relA] [protein|search|sasA] [protein|search|sasB]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[protein|search|guaB]'' or inactivation of ''[protein|search|codY]'' [Pubmed|24163341]
  • a ''[protein|search|relA] [protein|search|sasA] [protein|search|sasB]'' triple mutant acquires suppressor mutations in ''[protein|search|guaA], [protein|search|guaB], [protein|search|gmk]'' or inactivation of ''[protein|search|codY]'' [Pubmed|24682323,24163341]
  • mutation in ''[protein|search|relA]'' results in increased motility and chaining [Pubmed|25331430]
  • ''[protein|search|relA]'' mutation increases flagellin production via elevated [protein|search|SigD] level [Pubmed|25331430]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP GTP = AMP guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)
  • Protein family

  • relA/spoT family (according to Swiss-Prot)
  • [SW|Domains]

  • N-terminal ppGpp hydrolase domain [Pubmed|24163341]
  • central ppGpp synthetase domain [Pubmed|24163341]
  • Effectors of protein activity

  • the interaction with [SW|ComGA] inhibits the hydrolysis of ppGpp [Pubmed|25899641]
  • Structure

  • [PDB|1VJ7] (N-terminal catalytic fragment, from ''Streptococcus equisimilis'', 60% identity) [Pubmed|15066282]
  • [SW|Interactions]

  • [protein|search|RelA]-[SW|ComGA], required for the inhibition of rRNA synthesis in the K-state [Pubmed|25899641]
  • Expression and Regulation

    Operon

  • ''[protein|search|relA]-[protein|search|dtd]'' [Pubmed|9383190]
  • Biological materials

    Mutant

  • ''[protein|search|relA]'' mutant [Pubmed|9383190] - note that ''[protein|search|relA]'' mutants are prone to suppressor mutations in the ''[protein|search|ywaC]'' or ''[protein|search|yjbM]'' loci [Pubmed|18670626]
  • GP2066 (''[SW|sasA] [SW|sasB] [SW|relA]::mls''), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]
  • Jue D Wang, University of Wisconsin–Madison [https://www.researchgate.net/profile/Jue_Wang8 ResearchGate-Profile]
  • References

    Reviews

  • 27149325
  • Original publications

  • 25331430,9383190,10209741,24489751,19447912,13129942,12372825,12081964,11948165,12419222,23028324,24163341,24682323,25899641,15066282,27775002