SubtiBank SubtiBank
pyrG
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pyrG

CTP synthase (NH3, glutamine)
locus
BSU_37150
pI
5.16
mw
59.55 kDa
protein length
535 aa Sequence Blast
gene length
1608 bp Sequence Blast
function
pyrimidine biosynthesis
product
CTP synthase (NH3, glutamine)
essential
yes
ec
6.3.4.2
synonyms
ctrA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,810,693 → 3,812,300

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-glutamine + UTP --> ADP + CTP + 2 H+ + L-glutamate + phosphate (according to UniProt)
  • Protein family

  • CTP synthase family (single member, according to UniProt)
  • [SW|Domains]

  • [SW|Glutamine amidotransferase type-1 domain] (aa 292-534) (according to UniProt)
  • Structure

  • [PDB|1VCM] (from ''Thermus thermophilus'', 52% identity, 70% similarity) [Pubmed|15296735]
  • Expression and Regulation

    Operons

    genes
    [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]-[gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
    description
    [Pubmed|29794222]

    regulation

  • the mRNA is processed between [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE] and [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
  • view in new tab

    genes
    [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
    description
    [Pubmed|2457578]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15252202], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|RNA switch|RNA switch]: termination, in [regulon|RNA switch|RNA switch]
  • regulation

  • repressed by cytidine nucleotides ([SW|RNA switch]) [Pubmed|11544212]
  • view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
  • References

    Reviews

  • 21334966,18535147
  • Original publications

  • 8206849,17302819,15252202,11544212,1709162,12426364,17158658,2457578,28652344,26883633,29794222