trmR [2018-07-16 13:31:59]

tRNA wobble uridine modifying methyltransferase

locus: BSU27360

pI: 5.57

mw: 24.92 kDa

protein length: 217 aa

gene length: 651 bp

function: introduction of 5-methoxyuridine modification in tRNA

product: tRNA wobble uridine modifying methyltransferase

essential: no

synonyms: yrrM

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 2,795,082 → 2,795,735

The protein

Catalyzed reaction/ biological activity

• conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) [pubmed|29982645]

Protein family

• Type 3 family (according to Swiss-Prot)

Structure

• [PDB|5ZW3] [pubmed|29982645]
• [PDB|5ZW4] (bound to tRNA) [pubmed|29982645]

Expression and Regulation

Operons

• genes: [gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A853 (yrrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/853 NBRP B. subtilis, Japan]
• BKE27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
• BKK27360 (Δ[gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTACAAACAGCCTCCCGT, downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC

References

Research papers

• 29982645