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aapA

amino acid permease

locus: BSU_27090

pI: 9.68

mw: 50.49 kDa

protein length: 462 aa

gene length: 1389 bp

function: amino acid uptake

product: amino acid permease

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.2|Other amino acid transporters]

Gene

Coordinates: 2,766,558 → 2,767,946

The protein

Protein family

• [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|GabP], [protein|219586A3F378DC38EA076FD39B99D41136BDE722|YtnA], [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|HutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|YvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|YbxG], [protein|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|RocC], [protein|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|RocE], [protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|YbgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|YdgF]

Structure

• [PDB|3NCY] (from Salmonella typhimurium, 21% identity) [pubmed|19578361]

Expression and Regulation

Operons

• genes: [gene|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• GP2377 Δ''[gene|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA]''::''tet'', available in [SW|Jörg Stülke]'s lab
• MGNA-A230 (aapA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/230 NBRP B. subtilis, Japan]
• BKE27090 (Δ[gene|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT, downstream forward: _UP4_TAAAAAAAAGACAAGGGATA
• BKK27090 (Δ[gene|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTATTTTCCTCCAGT, downstream forward: _UP4_TAAAAAAAAGACAAGGGATA

Expression vector

• pGP2279: expression of ''aapA'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP2273 (in [SW|pAC5]) (GP2960), available in [SW|Jörg Stülke]'s lab

References

Research papers

• 19578361