SubtiBank SubtiBank
ureC
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ureC

urease (alpha subunit)
locus
BSU_36640
pI
5.09
mw
61.01 kDa
protein length
569 aa Sequence Blast
gene length
1710 bp Sequence Blast
function
utilization of urea as alternative nitrogen source
product
urease (alpha subunit)
essential
no
ec
3.5.1.5
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.2|Utilization of urea]
  • Gene

    Coordinates
    3,766,714 → 3,768,423

    The protein

    Catalyzed reaction/ biological activity

  • 2 H+ + H2O + urea --> CO2 + 2 NH4+ (according to UniProt)
  • Protein family

  • [SW|Metallo-dependent hydrolases superfamily] (according to UniProt)
  • [SW|Cofactors]

  • nickel
  • Structure

  • [PDB|5A6T] (from Sporosarcina pasteurii, 65% identity) [pubmed|26580226]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|63CF8C8BBCE39BD585DD5F6C8BBD95E12B500AE6|ywzF]-[gene|1D9596609AEF10456041918BD592E49466D93B1F|ywzE]-[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
    description
    [Pubmed|9287005]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9287005], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|9287005], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR]: repression, [Pubmed|9287005], in [regulon|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|9287005], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12374841], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|9287005,12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • regulation

  • induced by nitrogen limitation ([protein|search|GlnR], [protein|search|TnrA]) [Pubmed|9287005]
  • view in new tab

    Biological materials

    Mutant

  • BKE36640 (Δ[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCATATTCCTCCCGTGACA, downstream forward: _UP4_TGATAAGACGCGGCCGGAGT
  • BKK36640 (Δ[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCATATTCCTCCCGTGACA, downstream forward: _UP4_TGATAAGACGCGGCCGGAGT
  • References

    Reviews

  • 23539618,19363030
  • Original publications

  • 12618455,16199586,9287005,9287005,12374841,9287005,25755103,26580226