purS

phosphoribosylformylglycinamidine synthase

Locus: BSU_06460

Isoelectric point: 4.59

Molecular weight: 9.62 kDa

Protein length: 84 aa

Gene length: 255 bp

Function: purine biosynthesis

Product: phosphoribosylformylglycinamidine synthase

Essential: no

E.C.: 6.3.5.3

Synonyms: yexA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

Gene

Coordinates: 702,319 → 702,573

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ADP + H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• UPF0062 family (single member, according to UniProt)

Structure

• 1T4A

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

Biological materials

Mutant

• MGNA-A920 (yexA::erm), available at the NBRP B. subtilis, Japan
• BKE06460 (ΔpurS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC
• BKK06460 (ΔpurS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC

References

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