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gidB
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gidB

7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein
locus
BSU_41000
pI
6.60
mw
26.80 kDa
protein length
239 aa Sequence Blast
gene length
720 bp Sequence Blast
function
rRNA modification
product
7-methylguanosine methyltransferase specific for the 16S rRNA
essential
no
synonyms
rsmG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

    • Gene

    Coordinates
    4,208,870 → 4,209,589

    Phenotypes of a mutant

  • low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) [Pubmed|17573471]

    • The protein

    Catalyzed reaction/ biological activity

  • methylates the N7 position of nucleotide G535 in 16S rRNA [Pubmed|17573471]

    • Protein family

  • [SW|Methyltransferase superfamily] (according to UniProt)

    • Structure

  • [PDB|1XDZ]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]-[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]-[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]-[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
    description
    [Pubmed|11807071]

    regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [Pubmed|26020636], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
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    Biological materials

    Mutant

  • MGNA-B875 (gidB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1874 NBRP B. subtilis, Japan]
  • BKE41000 (Δ[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE41000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT
  • BKK41000 (Δ[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK41000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAATATTCATGCCGTCAT, downstream forward: _UP4_TAATTTAGACAGCCTGATAT

    • References

  • 17573471,11948146,11807071,26020636