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icd [Wed Aug 24 2016 13:11:11 GMT+0200 (CEST)]
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icd [Wed Aug 24 2016 13:11:11 GMT+0200 (CEST)]

isocitrate dehydrogenase
46.00 kDa
TCA cycle
isocitrate dehydrogenase

Genomic Context



  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins], [SW|phosphoproteins], [SW|most abundant proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CcpC regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,979,716 -> 2,980,987

    Phenotypes of a mutant

  • reduced ability to sporulate [Pubmed|9244258]
  • growth and sporulation defects of the mutant could be partially bypassed by deletion of the major citrate synthase gene (''[protein|search|citZ]'') [Pubmed|10348849]
  • The protein

    Catalyzed reaction/ biological activity

  • Isocitrate NADP = 2-oxoglutarate CO2 NADPH (according to Swiss-Prot)
  • Protein family

  • isocitrate and isopropylmalate dehydrogenases family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Arg-180 [Pubmed|22517742]
  • phosphorylated [Pubmed|17726680], [Pubmed|16493705]
  • ''in vitro'' phosphorylated by [protein|search|PrkC] on Thr-138, Thr-147, and Thr-396 [Pubmed|20389117]
  • [SW|Cofactors]

  • Mg2, Mn2, NADP
  • Effectors of protein activity

  • Inhibited by glyoxylate, oxaloacetate and oxalomalate [Pubmed|4147570]
  • Structure

  • [PDB|1HQS]
  • [SW|Localization]

  • attached to the membrane [Pubmed|18763711]
  • [SW|Interactions]

  • homodimer [Pubmed|11290745]
  • [protein|search|CitZ]-[protein|search|Mdh]-[protein|search|Icd] (this is the core of the TCA cycle metabolon) [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|Icd] [Pubmed|24325460]
  • [protein|search|GltA]-[protein|search|Icd] [Pubmed|20933603]
  • [protein|search|OdhB]-[protein|search|Icd] [Pubmed|20933603]
  • Additional information

  • This enzyme requires NADP exclusively. No activity was seen on the presence on NAD [Pubmed|4147570]
  • extensive information on the structure and enzymatic properties of Icd can be found at [ Proteopedia]
  • Expression and Regulation


  • ''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]'' [Pubmed|8045899]
  • ''[protein|search|icd]-[protein|search|mdh]'' [Pubmed|8045899]
  • [SW|Sigma factor]

  • ''[protein|search|citZ]'': [protein|search|SigA] [Pubmed|8045899]
  • ''[protein|search|icd]'': [protein|search|SigA] [Pubmed|8045899]
  • Regulation

  • ''[protein|search|citZ]'': catabolite repression ([protein|search|CcpA]) [Pubmed|12100558]
  • ''[protein|search|citZ]'': repression by glucose glutamate ([protein|search|CcpC]) [Pubmed|12100558]
  • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression, [protein|search|CcpC]: transcription repression [Pubmed|12100558]
  • [protein|search|CcpC]: transcription repression (molecular inducer: citrate) [Pubmed|10656796]
  • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Biological materials


  • GP666 (spc), GP672 (erm), available in [SW|Jörg Stülke]'s lab
  • 1A1000 ( ''icd''::''spec''), [Pubmed| ], available at [ BGSC]
  • 1A999 ( ''icd''::''spec''), [Pubmed| ], available at [ BGSC]
  • GP790 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP2331 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), cre-recombinase is integrated in ''[protein|search|sacA]'',available in [SW|Jörg Stülke]'s lab
  • GP2333 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''lox72''), cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP1121 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Linc Sonenshein]'s lab
  • Labs working on this gene/protein

  • [SW| Linc Sonenshein], Tufts University, Boston, MA, USA [ Homepage]
  • References

  • 10656796,12850135,11290745,10348849,11751849,18763711,17726680,16493705,4147570,20389117,9244258,20933603,22517742,24325460,24571712,15378759,24616559