era [Wed Jul 13 2016 14:04:17 GMT+0200 (CEST)]

GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

locus: BSU25290

pI: 8.00

mw: 33.00 kDa

function: ribosome assembly

product: GTP-binding protein

essential: yes

synonyms: bex, yqfH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes], [SW|GTP-binding proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigB regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,610,041 -> 2,610,946

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• Era subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

Effectors of protein activity

• binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]

Structure

• [PDB|3IEV] ([protein|search|Era] from ''Aquifex aeolicus'', 50% identity/ 77% similarity, complex with the 3' end of [SW|16S rRNA]) [Pubmed|19706445]

[SW|Interactions]

• [protein|search|Era]-[SW|16S rRNA] [Pubmed|19706445]

Expression and Regulation

Operon

• ''[protein|search|cdd]-[protein|search|era]'' (according to [http://dbtbs.hgc.jp/COG/prom/cdd-era.html DBTBS])

[SW|Sigma factor]

• SigA [Pubmed|6330116], SigB [Pubmed|6330116]

Biological materials

Expression vector

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP837, available in [SW|Stülke] lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP845, available in [SW|Stülke] lab

Labs working on this gene/protein

• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 19575570,21885683

Original publications

• 12427945,6330116,1970644526951678