bofA [2018-11-08 13:02:19]

inhibition of the pro-sigma-K processing machinery

locus: BSU00230

pI: 9.32

mw: 8.87 kDa

protein length: 87 aa

gene length: 261 bp

function: control of [protein|search|SigK] activation

product: inhibitor of pro-[protein|search|SigK] processing

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 29,772 → 30,035

The protein

[SW|Localization]

• forespore outer membrane (according to Swiss-Prot)
• integral membrane protein [Pubmed|11959848]

Expression and Regulation

Operons

• genes: [gene|73D063EDC412F7F28DA27C986ECCCD1C4A1F6618|dnaX]-[gene|9AFCEFE83560B1E2B1FFD7CFD2E4F17FAE137889|yaaK]-[gene|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]-[gene|C5127208E7562F09249112240F0BE8C582B2E5AD|yaaL]-[gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]
  description: [Pubmed|22383849]

• genes: [gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]
  description: [Pubmed|1315731]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|1315731], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|1315731], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed during sporulation [Pubmed|1315731]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-A081 (bofA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/81 NBRP B. subtilis, Japan]
• 1S100 ( ''bofA''::''cat''), [Pubmed|1577688], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S100&Search=1S100 BGSC]
• 1S96 ( ''bofA''::''erm''), [Pubmed|1315731], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S96&Search=1S96 BGSC]
• BKE00230 (Δ[gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTTCTCATCTCCT, downstream forward: _UP4_TAATTGATATTATGTATTGA
• BKK00230 (Δ[gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTTCTCATCTCCT, downstream forward: _UP4_TAATTGATATTATGTATTGA

labs

• Simon Cutting, London, UK [http://personal.rhul.ac.uk/uhba/009/bacillus/simon_cutting.html homepage]

References

• 11959848,9501233,1577688,15292188,2115401,12940997,12884008,15292188,1315731,15752199,16818230,15087499