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alr [Fri Apr 01 2016 18:17:52 GMT+0200 (CEST)]
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alr [Fri Apr 01 2016 18:17:52 GMT+0200 (CEST)]

locus
BSU04640
pI
8.00
mw
43.00 kDa
function
peptidoglycan biosynthesis
product
alanine racemase
essential
yes
ec
5.1.1.1
synonyms
dal, alrA

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]
  • Gene

    Coordinates on the chromosome (coding sequence)
    517,372 -> 518,541

    Phenotypes of a mutant

  • reported to be essential [Pubmed|12682299], however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007) [Pubmed|17602222]
  • auxotrophy for D-alanine
  • The protein

    Catalyzed reaction/ biological activity

  • L-alanine = D-alanine (according to Swiss-Prot)
  • Protein family

  • alanine racemase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YncD]
  • Structure

  • [PDB|1L6G] (with N-[5�-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus), [PDB|1SFT] (Geobacillus stearothermophilus)
  • Biological materials

    Mutant

  • [http://www.ncbi.nlm.nih.gov/nuccore/402479429?report=genbank&from=517383&to=518551 alr-1], two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in [http://www.bgsc.org/ BGSC] 1A4, a D-alanine auxotroph
  • References

  • Ferrari E, Henner DJ, Yang MY. Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis, Nature Biotechnol 1985.Nov;3:1003-7.
  • Nature Biotechnology 3, 1003–1007
  • 3138014
  • 17602222