SubtiBank SubtiBank
thrZ
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thrZ

threonyl-tRNA synthetase (minor)
Locus
BSU_37560
Isoelectric point
5.75
Molecular weight
73.20 kDa
Protein length
638 aa Sequence Blast
Gene length
1917 bp Sequence Blast
Function
translation
Product
threonyl-tRNA synthetase (minor)
Essential
no
E.C.
6.1.1.3
Synonyms
thrS2

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,854,256 → 3,856,172

The protein

Catalyzed reaction/ biological activity

  • ATP + L-threonine + tRNAThr --> AMP + diphosphate + H+ + L-threonyl-tRNAThr (according to UniProt)
  • Protein family

    Paralogous protein(s)

    Domains

  • TGS domain (aa 1-64) (according to UniProt)
  • Structure

  • 1TJE (from Escherichia coli, 43% identity, 65% similarity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • T-box: anti-termination, in T-box
  • Regulation

  • induced by threonine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • BKE37560 (ΔthrZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAGAACACTCCTTAT, downstream forward: _UP4_TAGGATAAAAGCTGTGAATT
  • BKK37560 (ΔthrZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAGAACACTCCTTAT, downstream forward: _UP4_TAGGATAAAAGCTGTGAATT
  • References

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