SubtiBank SubtiBank
pyrC
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pyrC

dihydroorotase
Locus
BSU_15500
Isoelectric point
5.43
Molecular weight
46.45 kDa
Protein length
428 aa Sequence Blast
Gene length
1287 bp Sequence Blast
Function
pyrimidine biosynthesis
Product
dihydroorotase
Essential
no
E.C.
3.5.2.3
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides

    • Gene

    Coordinates
    1,621,374 → 1,622,660

    The protein

    Catalyzed reaction/ biological activity

  • (S)-dihydroorotate + H2O --> H+ + N-carbamoyl-L-aspartate (according to UniProt)

    • Protein family

  • Metallo-dependent hydrolases superfamily (according to UniProt)

    • Paralogous protein(s)

  • PucH

    • Structure

  • 3GRI (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)

    • Expression and Regulation

    Operons

    Genes
    pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

    • Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed
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    Biological materials

    Mutant

  • BKE15500 (ΔpyrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC, downstream forward: _UP4_TATGAAGAGGGGAGACTTGT
  • BKK15500 (ΔpyrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC, downstream forward: _UP4_TATGAAGAGGGGAGACTTGT

    • References

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