cwlA

N-acetylmuramoyl-L-alanine amidase

locus: BSU_25900

pI: 10.40

mw: 29.81 kDa

protein length: 272 aa

gene length: 819 bp

function: minor autolysin

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.3|Skin element]

Gene

Coordinates: 2,664,573 → 2,665,391

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

Paralogous protein(s)

• [protein|D5612882F1887BE342EF7072E71502382AB7289F|CwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|XlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|XlyB]

[SW|Domains]

• contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|BlyA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|CwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|XlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|XlyB])
• [SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 24-142) (according to UniProt)

Structure

• [PDB|2L47] (from Bacillus phage Gamma, corresponds to the N-terminal domain, aa 3 ... 150, 61% identity)

Biological materials

Mutant

• BKE25900 (Δ[gene|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATACCCTCTCTCCTTCT, downstream forward: _UP4_TAAATAAATAGTCTCCTTGA
• BKK25900 (Δ[gene|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATACCCTCTCTCCTTCT, downstream forward: _UP4_TAAATAAATAGTCTCCTTGA

References

• 1908396