SubtiBank SubtiBank
pucA
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pucA

xanthine dehydrogenase
locus
BSU32510
pI
6.36
mw
36.59 kDa
protein length
330 aa Sequence Blast
gene length
993 bp Sequence Blast
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
yurF

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
  • Gene

    Coordinates
    3,339,945 → 3,340,937

    The protein

    Catalyzed reaction/ biological activity

  • Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Structure

  • [PDB|3ON5] (from B. halodurans, 44% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]
  • regulation

  • induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
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    Biological materials

    Mutant

  • MGNA-A965 (yurF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/965 NBRP B. subtilis, Japan]
  • BKE32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC, downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
  • BKK32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC, downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
  • References

  • 11344136,12029039,12823818