ydfH

two-component sensor kinase, control of [gene|1B61298ECA583D2112AA56A88358E8A9A55D2C9D|ydfJ] expression

locus: BSU_05410

pI: 9.60

mw: 46.34 kDa

protein length: 407 aa

gene length: 1224 bp

function: control of [gene|1B61298ECA583D2112AA56A88358E8A9A55D2C9D|ydfJ] expression

product: two-component sensor kinase (NarL family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 587,744 → 588,967

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|EFEAF09E4449A022A7323450FDED9458426A0080|YdfI]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• 4 transmembrane segments
• [SW|Histidine kinase domain] (aa 201-402) (according to UniProt)

Modification

• autophosphorylation on a His residue

Structure

• [PDB|5IUN] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK], C-terminal [SW|Histidine kinase domain], 28% identity) [pubmed|27938660]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|C86BAEE3DC97A96EFB85A0E2DF44AD69ED04744C|ydfH]-[gene|EFEAF09E4449A022A7323450FDED9458426A0080|ydfI]
  description: [Pubmed|15941986]

Biological materials

Mutant

• MGNA-C300 (ydfH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2298 NBRP B. subtilis, Japan]
• BKE05410 (Δ[gene|C86BAEE3DC97A96EFB85A0E2DF44AD69ED04744C|ydfH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCTTATCCACTTCCTT, downstream forward: _UP4_AAGATGAATAAGGTTTTAAT
• BKK05410 (Δ[gene|C86BAEE3DC97A96EFB85A0E2DF44AD69ED04744C|ydfH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCTTATCCACTTCCTT, downstream forward: _UP4_AAGATGAATAAGGTTTTAAT

References

• 10094672,15941986,27938660