SubtiBank SubtiBank
phoR
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phoR

two-component sensor kinase, regulation of phosphate metabolism
locus
BSU_29100
pI
5.96
mw
64.95 kDa
protein length
579 aa Sequence Blast
gene length
1740 bp Sequence Blast
function
regulation of phosphate metabolism
product
two-component sensor kinase
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • Gene

    Coordinates
    2,976,068 → 2,977,807

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
  • Paralogous protein(s)

  • [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK], [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE]
  • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain], for binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate)) [Pubmed|25315493]
  • [SW|Histidine kinase domain] (aa 357-575) (according to UniProt)
  • Modification

  • autophosphorylation on a His residue in response to the the availability of an intermediate of wall teichoic acid bioynthesis, autophosphorylation is prevented by binding of this intermediate to the intracellular [SW|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] [Pubmed|25315493]
  • Effectors of protein activity

  • activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate) to the intracellular [SW|PAS domain] of [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] [Pubmed|25315493]
  • the kinase activity is activated by an intermediate in the WTA biosynthetic pathway (product of [protein|72A89412C53703F96F835D26307263F5D1A4AB4E|TagA] or [protein|911A4D957A6C0DFC636A152395119DBFC777DDA2|TagB]) [pubmed|29644746]
  • Structure

  • [PDB|3CWF] (extracellular domain) [Pubmed|20008068]
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
    description
    [Pubmed|14762014]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452408], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15205429], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,15205429], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16452408,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|20382764], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, due to [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]~P binding to the 3’end of the [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]-[protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR] operon [Pubmed|25666134,15205429,14762014], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • regulation

  • carbon catabolite repression ([protein|search|CcpA]) [Pubmed|16452408,12850135]
  • view in new tab

    Biological materials

    Mutant

  • 1A966 ( ''phoR''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
  • BKE29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA
  • BKK29100 (Δ[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA
  • labs

  • [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
  • References

    Reviews

  • 10094672,25355628,30459743
  • Original publications

  • 20008068,10433720,15205429,9084179,17085571,9987123,10913081,16452408,20167622,15205429,14762014,20382764,25315493,29644746