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gabR [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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gabR [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

transcription activator of gabT-gabD, repressor of gabR (MocR/ GabR family)
locus
BSU03890
pI
6.00
mw
54.00 kDa
function
regulation of gamma-amino butyric acid utilization
product
transcription regulator
essential
no
synonyms
ycnF

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.10|Utilization of gamma-amino butyric acid]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids], [SW|transcription factors and their control]
  • This gene is a member of the following [SW|regulons]

  • [SW|GabR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    440,025 -> 441,464

    The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the ''[protein|search|gabT]-[protein|search|gabD]'' operon in the presence of GABA and PLP, transcription repression of ''[protein|search|gabR]'' [Pubmed|15223311]
  • Protein family

  • [[MocR/ GabR family]] [Pubmed|22020104]
  • Paralogous protein(s)

  • none, but there are 7 members of the [[MocR/ GabR family]] in ''B. subtilis
  • [SW|Domains]

  • N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]
  • C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574]
  • [SW|Cofactors]

  • gamma-aminobutyric acid, PLP [Pubmed|24127574]
  • Effectors of protein activity

  • activation of ''[protein|search|gabT]-[protein|search|gabD]'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate [Pubmed|15223311]
  • Structure

  • [PDB|4MGR] (the apo-protein) [Pubmed|24127574]
  • [PDB|4N0B] (the complex with PLP) [Pubmed|24127574]
  • [SW|Interactions]

  • forms head-to-tail domain-swapped dimers [Pubmed|24127574]
  • Expression and Regulation

    Operon

  • ''[protein|search|gabR]'' (according to [http://dbtbs.hgc.jp/COG/prom/gabR.html DBTBS])
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|12123465]
  • Regulatory mechanism

  • GabR: negative autoregulation [Pubmed|12123465]
  • Biological materials

    Mutant

  • MGNA-C014 (ycnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2012 NBRP B. subtilis, Japan]
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • References

  • 11756427,15223311,12123465,24127574,22020104,25388514,25911692,27640111