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wapI
immunity protein, protects the cell against the toxic activity of WapA
function
intercellular competition
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.18|Toxins, antitoxins and immunity against toxins/ based on similarity][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]Gene
Coordinates
4,023,054 → 4,023,482
Phenotypes of a mutant
essential [Pubmed|28189581]severe growth defect [Pubmed|20815827]The protein
Catalyzed reaction/ biological activity
[protein|C7400733DB8835A204A7FF26C87A92A15C5F318C|WapI] inhibits the toxic activity of [protein|BBC06DA57CAC2B2378763E0839C6C31AFBEA0417|WapA] [Pubmed|23572593]Modification
phosphorylation on (Tyr-102 OR Ser-105) [Pubmed|17218307]Expression and Regulation
Operons
genes
[gene|BBC06DA57CAC2B2378763E0839C6C31AFBEA0417|wapA]-[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]
description
[Pubmed|16306698]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23199363], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: repression, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|9537385], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon][protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon][protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: repression, [Pubmed|23199363], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]regulation
repressed at high salt concentration ([protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P) [Pubmed|32419322,9537385]Additional information
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]view in new tabBiological materials
Mutant
MGNA-B789 (yxxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1788 NBRP B. subtilis, Japan]BKE39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGCBKK39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGCReferences
16672620,20815827,17218307,16306698,9537385,23572593,23199363,26923784,28189581