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wapI

immunity protein, protects the cell against the toxic activity of WapA

locus: BSU39220

pI: 4.42

mw: 16.29 kDa

protein length: 142 aa

gene length: 426 bp

function: intercellular competition

product: immunity protein

essential: yes

synonyms: yxxG

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.18|Toxins, antitoxins and immunity against toxins/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 4,023,054 → 4,023,482

Phenotypes of a mutant

• essential [Pubmed|28189581]
• severe growth defect [Pubmed|20815827]

The protein

Catalyzed reaction/ biological activity

• [protein|C7400733DB8835A204A7FF26C87A92A15C5F318C|WapI] inhibits the toxic activity of [protein|BBC06DA57CAC2B2378763E0839C6C31AFBEA0417|WapA] [Pubmed|23572593]

Modification

• phosphorylation on (Tyr-102 OR Ser-105) [Pubmed|17218307]

Expression and Regulation

Operons

• genes: [gene|BBC06DA57CAC2B2378763E0839C6C31AFBEA0417|wapA]-[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]
  description: [Pubmed|16306698]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23199363], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: repression, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|9537385], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: repression, [Pubmed|23199363], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • repressed at high salt concentration ([protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P) [Pubmed|9537385]

  Additional information

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]

Biological materials

Mutant

• MGNA-B789 (yxxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1788 NBRP B. subtilis, Japan]
• BKE39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGC
• BKK39220 (Δ[gene|C7400733DB8835A204A7FF26C87A92A15C5F318C|wapI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGC

References

• 16672620,20815827,17218307,16306698,9537385,23572593,23199363,26923784,28189581